Journal: bioRxiv

Article Title: Sex differences in the neural circuitry of aggression

doi: 10.64898/2025.12.18.695285

Figure Lengend Snippet: DAPI-stained sections of other regions of the SBNN, as defined by the Hamster Brain Atlas (Wood and Morin, 2001). a) Representative section of BNST and POA at 4X. BSTP = posterior BNST, MPO = medial preoptic area, MPN = medial preoptic nucleus, ac = anterior commissure. b) Representative section of VMH at 4X. DM = dorsomedial hypothalamic nucleus, f = fornix, Mtu = medial tuberal nucleus. c) Representative section of the PVN, AH, mSON, and LH. 3V = third ventricle, f = fornix, SCN = suprachiasmatic nucleus, sox = supraoptic decussation, ox = optic chiasm. d) Representative section of the amygdala at 4X. ot=optic tract, pir = piriform cortex, MGP= medial globus pallidus. e) Representative section of the DR and PAG (DMPAG, DLPAG, LPAG) at 4X. DMPAG = dorsomedial PAG, DLPAG = dorsolateral PAG, LPAG = lateral PAG, *=aqueduct, 4V = fourth ventricle, LDTg = lateral dorsal tegmental nucleus

Article Snippet: Sections were mounted onto Super Frost Plus slides and coverslipped with Vectashield Hard Set Mounting Medium for Fluorescence with DAPI (H1500, Vector Laboratories, Burlingame, CA).

Techniques: Staining

Journal: bioRxiv

Article Title: Sex differences in the neural circuitry of aggression

doi: 10.64898/2025.12.18.695285

Figure Lengend Snippet: Representative images of anterior and posterior lateral septum (LS) subregions at 4X magnification. a,e,f) Representative image of the dorsal (ROI: 0.482 mm 2 ), intermediate (ROI: 0.359 mm 2 ), and ventral (ROI: 0.767 mm 2 ) subregions of the anterior LS. b,d) Schematic illustration depicts the dorsal (ROI: 0.721 mm 2 ), intermediate (ROI:1.023 mm 2 ), and ventral (ROI:0.892 mm 2 ) subregions of the anterior and posterior LS at +1.55LJmm (anterior) and +0.8LJmm (posterior) from Bregma (adapted from ). c,g,h) Representative image of the dorsal, intermediate, and ventral subregions of the posterior LS with DAPI (blue) and c-Fos (red). dLS=dorsal LS, iLS = intermediate LS, vLS = ventral LS, MS = medial septum, ac = anterior commissure, gcc=genu of the corpus collosum, SHi = septohippocampal nucleus, AcbSh = nucleus accumbens shell, AchC = nucleus accumbens core, BSTAM = anteromedial part of the bed nucleus of the stria terminalis (BNST), BSTAI = anterointermediate part of the BNST, BSTAV = anteroventral part of the BNST, LV = lateral ventricle.

Article Snippet: Sections were mounted onto Super Frost Plus slides and coverslipped with Vectashield Hard Set Mounting Medium for Fluorescence with DAPI (H1500, Vector Laboratories, Burlingame, CA).

Techniques:

Journal: bioRxiv

Article Title: IL-13 Modulates Antiviral Effector and Proinflammatory Pathways in Rhinovirus-Infected Pediatric Bronchial Epithelium

doi: 10.64898/2025.12.06.692776

Figure Lengend Snippet: (A) Schematic of the experimental design. Bronchial brushings were collected from children (n=61) under anesthesia for elective procedures. Cells were differentiated at an air-liquid interface culture for 21 days, then treated with IL-13 (10ng/mL) for 7 days prior to apical infection with human rhinovirus A16 (RV-A16) at an MOI 0.5. IL-13 treatment was continued through the infection timepoints. (B) H&E (top panels) and immunofluorescence (bottom panels) microscopy of bronchial epithelial cells in air-liquid interface cultures untreated and treated with IL-13 (10ng/mL). Immunofluorescence key: Blue = DAPI, Green = Tubulin, Red = MUC5AC. (C) Scanning electron microscopy (SEM) images of BECs untreated and treated with IL-13 (10ng/mL). (D) transmission electron microscopy (TEM) image of goblet cell degranulating mucus.

Article Snippet: Coverslips were mounted using Vectashield fluorescent mounting medium with DAPI (Vector Laboratories).

Techniques: Infection, Immunofluorescence, Microscopy, Electron Microscopy, Transmission Assay